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Project Descriptions and Photos

Honey Bee Research:

The preservation of genetic diversity is important for healthy species. This diversity is rapidly dwindling because of a series of recent plagues affecting honeybees. We work to preserve genetic diversity by developing methods for freezing honeybee semen in liquid nitrogen for long-term storage. We are also working on the cryopreservation of honeybee embryos. These technologies will need to be rapidly implemented. We will do that work also.

» View Project Poster - Cryopreservation of Honey Bee Spermatozoa

Universal Reproductive Technologies:

Developing a wide range of universally applicable reproductive technologies as well as cryobiology techniques; the aim is to make it so that each type of technology does not have to be repeatedly developed for different species.

» View Project Poster - Suitability Of Bovine Sexing Primers For Sex Determination Of Caprine Cells

» View Project Poster - Still Glowing After All These Years: Storage Life of a Complete Bovine Sexing PCR Mix

Frog Research:

The development of in-vitro fertilization methods; amazingly little is currently known about how to artificially reproduce frogs.  We are also working on sperm and egg collection and cryopreservation for species preservation.

» View Project Poster - Cryopreservation of Frog (Rana Pipiens) Sperm Cells Collected By Non-lethal Methods

Bird Reproductive Technologies:

Little research has been focused on endangered species research in avian species. So far, we have developed a rapid molecular-based sexing assay. We have also succeeded in egg transplantation. Further research will create powerful tools for bird reproduction.

» View Project Poster - The Enhancement of the Sensitivity Of A PCR-based Avian Sex Determination Assay

Robotic Sperm Injection:

Often sperm can be stored in liquid nitrogen and when thawed, it is still alive. However, the sperm cells are no longer motile.  We are working on a robotic system to inject the sperm into the eggs. Upon completion, within each hour several thousand eggs will be injected.

» View Project Poster - A Hybrid Approach for Modeling and Assessing Performance of Embedded Systems in a Biologically Critical Application

Gene Banking:

Storing the genetics of many individuals of a wide range of animals is essential for long-term survival of species.  As more species are becoming endangered and extinct, it is extremely important that we protect and preserve the genetic information we still have before it is too late. The dilemma with condors, leopards, and other small population restoration efforts is that because they began late, and the surviving population size was small, there is now too little genetic diversity. These creatures will never be as healthy as if the genetic diversity had been protected. If semen samples from many individuals had been preserved before the population declined, this problem could have been successfully avoided. It takes thousands of years to accumulate the genetic diversity of a typical healthy population.  We are currently working on honeybees, fish and amphibians, and will continue with many more species in the future.

Real World Scale Up:

Dissemination and large-scale implementation of reproductive technologies.  Unlike many research projects that simply end up in a dusty book stack in the library of a university, we intend to put our research results into real-world use.  We have the expertise to do this because of our knowledge of the artificial breeding industry.

Large Quantity Fish Semen Freezing Technology:

Unlike some public officials who claim that salmon are salmon, the genetics of a salmon dictate their adaptability to a particular environment.  Some sub-species are able to swim 200 miles to spawn, while others are able to migrate as far as 2000 miles to their spawning grounds.  Wild salmon genetics is rapidly vanishing.  We wish to preserve as much genetic diversity in salmon as possible as quickly as we can.  To do so we must first adapt semen freezing technology to work on far larger volumes than have been used before.  We have a plan for this scale-up.  Next we must implement the technology quickly.



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